Anti-RNA polymerase II CTD (phospho Ser7) Antibody [3D4A12]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

RNA polymerase II CTD

Applications

IF, WB

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rat

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

IF: 2 µg/ml - 10 µg/ml, WB: 0.5 µg/ml - 2 µg/ml

Clonă

3D4A12

Imunogen

A peptide containing the RNA pol II CTD sequence phosphorylated at serine 7.

Specificitate

This antibody recognises RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at serine 7 of the carboxyl-terminal repeat domain.RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA (Kornburg, 1999). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle. Phosphorylation of the CTD of RNA pol II at serine 7 is found on all pol II-transcribed genes in mammalaian cells, however, this event is only essential for expression of a sub-class of genes encoding small nuclear (sn) RNAs. It is thought that phosphorylation of the CTD of RNA pol II at serine 7 facilitates transcription of these genes and 3' end processing of the transcripts, through recruitment of the RPAP2 phosphatase and the snRNA gene-specific Integrator complex (Egloff, 2012).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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