Anti-Histone H3.1 (phospho Ser28) Antibody [5D10D4]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Histone H3.1

Applications

IF, WB

Reactivity

Human, Broad

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rat

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

IF: 2 µg/ml - 10 µg/ml, WB: 0.5 µg/ml - 2 µg/ml

Clonă

5D10D4

Imunogen

A peptide containing phosphorylated serine 28 of human histone H3.1.

Specificitate

This antibody recognises Histone H3.1 when phosphorylated at serine 28.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). In contrast to histone H3.3, incorporation of histone H3.1 and histone H3.2 into nucleosomes is replication dependent rather than occurring throughout the cell cycle. Phosphorylation of histone H3.1 at serine 28 occurs in early mitosis and during premature chromosome condensation in cells that are in S-phase of the cell cycle. Rat anti Human histone H3.1 (pSer28) antibody, clone 5D10D4 does not react with histone H3.3 phosphorylated on serine 28. Wide species cross-reactivity is expected based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

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