Anti-RNA polymerase II CTD (phospho Ser2 + Ser5) Antibody [1A12G10]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

RNA polymerase II CTD

Applications

WB, IF, ChIP

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

ChIP: 5 µg per ChIP - 10 µg per ChIP, IF: 5 µg/ml, WB: 0.5 µl/ml - 2 µl/ml

Clonă

1A12G10

Imunogen

Synthetic peptide of phosphorylated serine 2 and phosphorylated serine 5 of the CTD of human RNA polymerase II.

Specificitate

This antibody recognises RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at serine 2 or serine 5 of the carboxyl-terminal repeat domain.RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA (Kornburg, 1999). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle. Repeats phosphorylated on both serine 2 and serine 5 are required for optimal binding of the SRI domain of the methyl transerase SET2 to RNA pol II. Wide species cross-reactivity is expected from Mouse anti Human RNA pol II CTD (pSer2/pSer5), clone 1A12G10 based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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