Anti-RNA polymerase II CTD (phospho Thr4) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

RNA polymerase II CTD

Applications

WB

Reactivity

Human, Broad

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

WB: 1 µg/ml - 2 µg/ml

Imunogen

A peptide containing the RNA pol II C-terminus sequence phosphorylated at threonine 4.

Specificitate

This antibody recognises RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at threonine 4 of the carboxyl-terminal repeat domain.RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA (Kornburg, 1999). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle. Phosphorylation of the CTD of RNA pol II at threonine 4 has been shown to facilitate histone mRNA 3' end processing. Phosphorylation of the carboxyl-terminal repeat domain of RNA polymerase II at threonine 4 occurs at the 3' end of most human genes and is necessary for transcription elongation. Wide species cross-reactivity is expected from Rabbit anti Human RNA pol II CTD (pThr4) based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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