Anti-Histone H3.1 Antibody [1D4F2]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Histone H3.1

Applications

IF, WB

Reactivity

Human, Broad

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

IF: 0.4 µg/ml - 2 µg/ml, WB: 0.2 µg/ml - 2 µg/ml

Protein Length

19

Clonă

1D4F2

Secvență

LATKAARKSAPATGGVKKP

Imunogen

A peptide corresponding to amino acids 21-39 of human histone H3.1. This region is 100% identical in human histone H3.2.

Aminoacizi

aa 21 - 39

Specificitate

This antibody recognises Histone H3 variant H3.1.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). In contrast to histone H3.3, incorporation of histone H3.1 and H3.2 into nucleosomes is replication dependent rather than occurring throughout the cell cycle.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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