Anti-RNA polymerase II CTD (phospho Ser5) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

RNA polymerase II CTD

Applications

ChIP, WB

Reactivity

Human, Broad, Mouse

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

ChIP: 4 µg per ChIP - 10 µg per ChIP, WB: 0.5 µg/ml - 2 µg/ml

Imunogen

Synthetic peptide corresponding to a region within the C-terminus of RNA polymerase II phosphorylated at serine 5.

Specificitate

This antibody recognises RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at serine 5 of the carboxyl-terminal repeat domain.RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA (Kornburg, 1999). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle. It is thought that phosphorylation of the CTD of RNA pol II at serine 5 facilitates the formation of 3' ends of messages. Phosphorylation of serine 5 of the carboxyl-terminal repeat domain of RNA pol II facilitates the recruitment of the capping enzyme. Wide species cross-reactivity is expected from Rabbit anti Human RNA pol II CTD (pSer5) based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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