Anti-POLR3A Antibody [AB01/1E10]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

POLR3A

Applications

WB, IP

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

WB: 1:1,000

Protein Length

397

Clonă

AB01/1E10

Secvență

IFSVILRPSDDNPVRANLRTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVIRDHAGSACLRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPAAMEGKDEPLEFKRVLDNIKAVFPCPSEPALSKNELILTTESIMKKSEFLCCQDSFLQEIKKFIKGVSEKIKKTRDKYGINDNGTTE

Imunogen

E. coli derived recombinant protein corresponding to amino acids 600-996 of human POLR3A.

Aminoacizi

aa 600 - 996

Specificitate

This antibody recognises POLR3A, otherwise known as DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase III subunit A, RNA polymerase III 155kDa subunit and RNA polymerase III subunit C160.DNA-directed RNA polymerase is responsible for catalyzing the transcription of DNA into RNA via the four ribonucleoside triphosphates as substrates. It constitutes the largest and catalytic core of RNA polymerase III and transcribes small RNAs such as 5S rRNA and tRNAs; molecules essential for the normal functioning and survival of cells. This protein plays a critical role in detecting and controlling infection by intracellular bacteria and DNA viruses. It also acts as a non-self double stranded DNA sensor in the innate immune response. Synthesis of non–self DNA into RNA leads to type I interferon production via the RIG-1 pathway. Mutations in the POLR3A gene have been linked to a cluster of inherited neurodegenerative disorders called Hypomyelinating Leukodystrophy. Furthermore, studies show the possible connection of the mutated POLR3A gene to autoimmune diseases such as Scleroerma. Mouse anti Human POLR3A antibody detects a band of 157 kDa.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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