Anti-TIM 4 Antibody [ROS-JH9]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

TIM 4

Applications

Flow Cytometry

Reactivity

Chicken

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Protein Length

209

Clonă

ROS-JH9

Imunogen

Recombinant Chicken TIM-4 extracellular domain (amino acids 1-209) fused to chicken IgFc region.

Aminoacizi

aa 1 - 209

Specificitate

This antibody recognises chicken TIM-4 (T-cell immunoglobulin mucin 4).In mice, TIM-4 is largely expressed on antigen-presenting cells and involved in phagocytosis of apoptotic cells with, additionally, a regulatory role in T-cell responses. In chickens, the gene for TIM-4 codes for a minimum of five splice variants that vary between stains. Myeloid antigen-presenting cells and ?d T cells express chTIM4; in contrast to mammalian T cells which do not express TIM-4. Research conducted by Hu et al. using Mouse anti Chicken TIM-4 antibody, clone ROS-JH9, revealed that it can detect all isoforms of chicken TIM-4. Clone ROS-JH9 was found to stain bone marrow macrophages from J line birds, a cell population that was also positive for clone KUL01 MCA5770. Further work characterized a distinct TIM-4 bearing set of phagocytes in the bursa and showed that approximately 50% of blood monocytes in chicken express detectable levels of TIM-4.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului