Anti-Siglec 10 Antibody [2E9]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Siglec 10

Applications

Flow Cytometry

Reactivity

Porcine

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:200 - 1:1,000, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Clonă

2E9

Imunogen

NIH/3T3 mouse cells transfected with plasmid pSiglec-10-GFP, encoding the porcine Siglec-10 tagged with the green fluorescent protein (GFP) on the carboxy-terminal end.

Specificitate

This antibody recognises Siglec-10.Sialic-acid-binding immunoglobulin-like lectin (Siglecs) are cell surface receptors belonging to the immunoglobulin superfamily and recognize terminal sialic acids present in complex oligosaccharides of glycoproteins or glycolipids. Siglecs are mainly expressed on the cells of the immune systems and play a regulatory role, modulating inflammatory and immune responses. When expressed on transfected cells, porcine Siglec-10 is able to bind red blood cells in a sialic acid-dependent manner. Mouse anti Pig Siglec-10 antibody, clone 2E9 has been successfully used to examine Siglec-10 cell and tissue distribution in swine. Siglec-10 was found to be expressed on blood B cells (CD21+ or CD79a+) and in B cell areas of the spleen and lymph nodes. Weak expression was also detected on monocytes.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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