Tyramide Signal Amplification Kit - 5 Color

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Descriere

Specificații

Brand

Antibodies

Mărime

250 Reactions

Applications

IHC, ICC/IF, Multiplex IF, Sequential IHC, TSA Amplification

Conjugate

Fluorescent Tyramide Dyes

Product form

Lyophilized Powder and Liquid

Recommended dilutions

Recommended tyramide dye working dilution range: 1:50–1:200 depending on desired signal intensity and optimization requirements.

Assay type

Tyramide Signal Amplification Kit

Sample type

FFPE Tissue Sections, Frozen Tissue Sections, Tissue Microarrays (TMA), Cultured Cells, Microscope Slides

Detection type

Fluorescent TSA Detection

Platform

Multiplex IHC, Immunofluorescence, Sequential TSA Staining

Assay Time

Typical TSA color development time: 10–20 minutes per staining cycle.

Principiul testului

Tyramide signal amplification (TSA) is a highly sensitive fluorescent method for amplifying immunohistochemical staining when using HRP-conjugated antibodies. After applying HRP-labeled antibody to the sample and washing as in a normal IHC workflow, the tyramide fluorochrome is diluted in tyramide amplification buffer and applied to the samples for 10 minutes to develop the fluorescence. This is then repeated for multiple HRP-labeled antibodies by quenching HRP (HRP-block provided in StreptaClick® HRP Multiplex IHC Kit) and repeating the process for each antibody in sequence.

Storage

Shipped at +4°C. Store in the dark at -20°C. Before use, dissolve each vial in 100µl DMSO + 100µl PBS. Aliquot in vials and store at -20°C. The aliquots can be stored in the freezer for at least 12 months. Once thawed, store at +4°C and use within a week.