StreptaClick® HRP Complete Tyramide Signal Amplification and Biotinylation Kit - 3 Color

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Descriere

Specificații

Brand

Antibodies

Mărime

250 Reactions

Applications

IHC, ICC/IF, Multiplex IF, Sequential IHC, TSA Amplification

Conjugate

HRP-Conjugated Monovalent Streptavidin

Product form

Liquid and Resin

Assay type

Complete Multiplex TSA Immunohistochemistry Kit

Sample type

FFPE Tissue Sections, Frozen Tissue Sections, Tissue Microarrays (TMA), Cultured Cells, Microscope Slides

Detection type

Fluorescent TSA Detection

Platform

Multiplex IHC, Immunofluorescence, Sequential TSA Staining

Assay Time

Same-day multiplex staining workflows achievable.

Recovery

High-efficiency multiplex TSA staining with preserved tissue morphology and minimized antibody aggregation.

Specificitate

Complete multiplex TSA staining workflow for up to 3-color fluorescent immunohistochemistry using HRP-conjugated biotinylated antibodies.

Principiul testului

StreptaClick® HRP Complete Tyramide Signal Amplification and Biotinylation Kit provides everything needed for highly sensitive, multiplex IHC with three different antibodies. First, this kit offers a rapid, robust, and flexible method to generate horseradish peroxidase-conjugated primary antibodies using click chemistry, compatible with primary antibodies from any host species. Second, the novel HRP block buffer provided efficiently quenches HRP activity between staining cycles. Unlike other buffers, this HRP block buffer does not require boiling between cycles of immunostaining, which usually destroys frozen sections. This makes StreptaClick® HRP compatible with both FFPE sections and frozen sections, preserving tissue morphology while reducing the time for each staining cycle. Finally, the tyramide signal amplification (TSA) kit provided enables highly sensitive multiplexed staining by sequential IHC within a single day. StreptaClick® labeling occurs within 10 minutes, is highly efficient and can be carried out at any volume, concentration, or buffer. Once conjugated to HRP, each antibody is sequentially added to the sample and developed using tyramide signal amplification reagents. Quenching of each antibody-HRP conjugate with the novel HRP blocking buffer allows subsequent antibodies to be added in turn, until tyramide signal amplification has been performed for all antibodies.

Storage

Shipped at +4°C. Store in the dark at +4°C. Avoid prolonged exposure to light. Do not freeze!