StreptaClick® Antibody Labeling and Biotinylation Kit - 2 Color

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

2 x 500µg

Applications

IHC, ICC/IF, Multiplex IF, Flow Cytometry, FACS

Reactivity

Fluorochrome-Conjugated Monovalent Streptavidin

Formulation

The StreptaClick® antibody labeling reagent contains 0.09% Sodium Azide.

Product form

Liquid and Resin

Purification

Monovalent streptavidin labeling minimizes antibody aggregation during fluorochrome conjugation.

Recommended dilutions

Each labeling reagent contains 500 µl StreptaClick® reagent capable of labeling 50 µg biotinylated antibody, sufficient for approximately 50 slides.

Assay type

Antibody Labeling Kit

Sample type

FFPE Tissue Sections, Frozen Tissue Sections, Tissue Microarrays (TMA), Cultured Cells, Cytospins, Cell Suspensions, Microscope Slides

Detection type

Fluorescent Detection

Platform

Multiplex IHC, Immunofluorescence, Flow Cytometry

Assay Time

Approximately 5 minutes labeling time

Reconstitution

Ready-to-use labeling reagent supplied in solution.

Recovery

Stable fluorochrome-labeled antibodies with minimal aggregation and high labeling efficiency.

Specificitate

Monovalent streptavidin-based fluorochrome labeling of biotinylated antibodies without antibody aggregation.

Principiul testului

StreptaClick® Antibody Labeling and Biotinylation Kits provide a rapid, robust, and flexible method to conjugate fluorochromes to antibodies after biotinylation using click chemistry. Once labeled with a fluorochrome, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. StreptaClick® labeling occurs within 5 minutes, is highly efficient and can be carried out at any volume, concentration, or buffer. The antibody-fluorochrome conjugates are then mixed together in a single tube with the Biotin Block Buffer provided, after which they are ready to be used as primary antibodies in a normal IHC or flow cytometry workflow. StreptaClick®-antibody conjugates are compatible with DAPI, other directly conjugated primary antibodies and conventional secondary antibodies in the same assay. StreptaClick® is not compatible with other streptavidin-based techniques.

Storage

Shipped at +4°C. Upon delivery, open immediately and store specific components at -20°C and others at +4°C according to product manual. Avoid exposure to light!