StreptaClick® HRP Multiplex Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

90 Reactions · 250 Reactions

Applications

IHC, ICC/IF, Multiplex IF, Sequential IHC, TSA Amplification

Conjugate

HRP-Conjugated Monovalent Streptavidin

Product form

Liquid

Assay type

Multiplex TSA Immunohistochemistry Kit

Sample type

FFPE Tissue Sections, Frozen Tissue Sections, Tissue Microarrays (TMA), Cultured Cells, Microscope Slides

Detection type

Fluorescent TSA Detection

Platform

Multiplex IHC, Immunofluorescence, Sequential TSA Staining

Assay Time

Same-day multiplex staining workflows achievable for up to 6 markers plus DAPI.

Recovery

High-efficiency HRP labeling with preserved tissue morphology during sequential staining cycles.

Specificitate

Monovalent streptavidin-mediated HRP labeling of biotinylated primary antibodies for multiplex TSA staining workflows.

Principiul testului

StreptaClick® HRP Multiplex IHC Kits provide a rapid, robust, and flexible method to conjugate horseradish peroxidase (HRP) to biotinylated antibodies using click chemistry, and a novel HRP block buffer that efficiently quenches HRP activity between staining cycles. Unlike other buffers, this HRP block buffer does not require boiling between cycles, making it compatible with both FFPE sections and frozen sections, reducing the time for each staining cycle, and preserving tissue morphology. In combination with the Tyramide Signal Amplification Kit - 3 Color or Tyramide Signal Amplification Kit - 5 Color, this kit enables highly sensitive multiplexed staining by sequential IHC within a single day. StreptaClick® labeling occurs within 10 minutes, is highly efficient and can be carried out at any volume, concentration, or buffer. Once conjugated to HRP, each antibody is sequentially added to the sample and developed using tyramide signal amplification reagents. Quenching of each antibody-HRP conjugate with the novel HRP blocking buffer allows subsequent antibodies to be added in turn, until tyramide signal amplification has been performed for all antibodies.

Storage

Shipped at +4°C. Store in the dark at +4°C. Avoid prolonged exposure to light. Do not freeze!