Biotinylation Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

5 Reactions

Applications

IHC, ICC/IF, Flow Cytometry, ELISA, WB, IP

Product form

Lyophilized Powder, Liquid and Resin (Spin Columns)

Purification

Spin column purification with wash steps for removal of excess free biotin.

Recommended dilutions

Use with 25–100 µg antibody per reaction. Recommended minimum antibody concentration: 0.2 mg/ml for 100 µg reactions.

Assay type

Antibody Labeling Assay

Sample type

Antibodies, Purified Antibodies, Primary Antibodies, Cell Lysates, Tissue Lysates, Serum, Plasma

Detection type

Biotin/Streptavidin Detection

Platform

IHC, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation

Assay Time

Approximately 1 hour

Reconstitution

Dissolve Biotinylation Reagent in 100 µl DMSO prior to use.

Specificitate

Designed for biotinylation of purified carrier protein-free antibodies using amine-reactive NHS ester chemistry.

Principiul testului

The Biotinylation Kit an efficient method of generating highly pure biotinylated antibodies. Your chosen antibody is first mixed with the reaction buffer and immobilized to resin within a spin column. The biotinylation reagent is then added and the antibody biotinylation reaction takes place on the resin. After washing, the pure biotinylated antibody is eluted from the resin, with the whole process taking approximately 1 hour. The kit is compatible with antibodies from any host species.

Storage

Shipped at +4°C. Upon delivery, open immediately and store Biotinylation Reagent at -20°C. All other contents can be stored at room temperature.