Imagine indisponibilăAntibodiesA326318
Universal 27-Hydroxycholesterol ELISA Kit
3.993,00 lei3.300,00 leiTVA inclus (21%)fără TVA
Universal 27-Hydroxycholesterol ELISA Kit
Pe scurt
Universal 27-Hydroxycholesterol ELISA Kit este un kit ELISA de tip Competitive (quantitative) pentru măsurarea 27-Hydroxycholesterol, cu reactivitate declarată pentru Universal. Măsoară în domeniul 15.625-1000 pg/ml, are o sensibilitate de 9.375 pg/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 3.746,77 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Universal 27-Hydroxycholesterol ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal 27-Hydroxycholesterol in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Applications
- Competitive ELISA
- Reactivity
- Universal
- Assay type
- Competitive (quantitative)
- Sensitivity
- 9.375 pg/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for 27-Hydroxycholesterol with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 15.625-1000 pg/ml
- Assay Time
- 2 hours
- Cas number
- CAS:20380-11-4
- Principiul testului
- Universal 27-Hydroxycholesterol ELISA Kit (High Sensitivity) (A335444) is a 2 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal 27-Hydroxycholesterol in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with 27-Hydroxycholesterol. During the assay, 27-Hydroxycholesterol present in the standards or samples competes with immobilized 27-Hydroxycholesterol for binding to an assay-specific antibody. Following incubation, unbound components are removed by washing and HRP-Streptavidin conjugate is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of 27-Hydroxycholesterol in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.
Documentație
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