Universal Estradiol ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Estradiol

Applications

Competitive ELISA

Reactivity

Universal

Assay type

Competitive (quantitative)

Sensitivity

7.5 pg/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for Estradiol with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

12.5-800 pg/ml

Assay Time

2 hours

Principiul testului

Universal Estradiol ELISA Kit (A335449) is a 120 minute competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal Estradiol in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Estradiol. During the assay, Estradiol present in the standards or samples competes with a fixed amount of assay-specific detection reagent for binding to the immobilized antibody. Following incubation, unbound components are removed by washing and HRP-conjugated detection reagent is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of Estradiol in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.