Human Granzyme B ELISpot Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Granzyme B

Applications

ELISpot

Reactivity

Human

Conjugate

Unconjugated

Sample type

Peripheral blood mononuclear cells (PBMCs).

Detection type

Colorimetric

Platform

Pre-coated Microplate (1 x 96)

Assay Time

3h after cell stimulation

Specificitate

This product recognizes natural human Granzyme B.

Principiul testului

Human Granzyme B ELISpot Kit (A341817) is a sandwich ELISA designed for the sensitive and quantitative measurement of human Granzyme B in peripheral blood mononuclear cells (pbmcs). The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for Granzyme B. Standards and samples are added to the wells, allowing Granzyme B present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for Granzyme B is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of Granzyme B in the sample. The concentration of Granzyme B is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Storage

Store at 4°C.