Human Granzyme B ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Granzyme B

Applications

ELISA

Reactivity

Human

Formulation

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

Host

Rabbit

Product form

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Assay type

Sandwich (quantitative)

Sensitivity

39.826 pg/ml

Sample type

Serum, plasma and cell culture supernatant.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

78.13 pg/ml - 5000 pg/ml

Assay Time

1 hour

Recovery

Cell culture supernatant: 112%; Serum: 103%; Plasma: 101%

Imunogen

The immunogens for the antibodies in this kit are recombinant Granzyme B.

Principiul testului

Human Granzyme B ELISA Kit (A334814) employs a one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of human Granzyme B in serum, plasma, and cell culture supernatant. A 96-well microtiter plate is provided pre-coated with an antibody specific for Granzyme B. Standards and test samples are added to the wells, allowing Granzyme B to bind to the immobilized capture antibody. A prepared antibody mixture is added and incubated to form antibody–antigen complexes. After incubation, unbound material is removed by washing, and TMB substrate is added to develop colour through an HRP-catalysed reaction. The reaction is stopped with acidic stop solution, producing a yellow endpoint signal. The intensity of the colour is proportional to the amount of Granzyme B captured in each well. The concentration of Granzyme B is determined by measuring absorbance at 450 nm and interpolating from the standard curve.

Storage

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.