Anti-Histone H3 (phospho Thr32) Antibody [6C7G12]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Histone H3

Applications

WB

Reactivity

Human, Broad

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rat

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

WB: 1 µg/ml - 2 µg/ml

Clonă

6C7G12

Imunogen

A peptide containing phosphorylated threonine 32 of human histone H3.1.

Specificitate

This antibody recognises Histone H3 when phosphorylated at threonine 32.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation. Phosphorylation of histone H3 at threonine 32 has been shown to be enhanced in cells undergoing mitosis and is thought to play a distinct role due to its unique distribution across chromosomes compared to other histone H3 phosphorylation events. Wide species cross-reactivity is expected from Rat anti Human histone H3 (pThr32) antibody based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului