Anti-Granulocytes Antibody [6D10] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Granulocytes

Applications

Flow Cytometry

Reactivity

Porcine

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Clonă

6D10

Imunogen

Porcine bone marrow haematopoietic cells (BMHC).

Specificitate

This antibody recognises a ~60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2 (MCA2600), allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+ mature neutrophils and 6D10-2B2+ mature eosinophils and basophils. Mouse anti Pig Granulocytes antibody, clone 6D10 has been shown as suitable for use on cytospins (Pérez et al. 2007).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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