Anti-MICA + MICB Antibody [6D4] - BSA and Azide free

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

500µg

Țintă

MICA + MICB

Applications

IHC-Fr, Flow Cytometry, IP, Functional Assays

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: 1:50 - 1:200, IHC-Fr: 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Clonă

6D4

Imunogen

MICA transfected C1R cells.

Specificitate

This antibody recognises non-classical MHC class I chain A (MICA) and non-classical MHC class I chain MICB (MICB). MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.

Storage

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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