Vomitoxin/Deoxynivalenol ELISA Kit

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Specificații

Brand

Antibodies

Mărime

96T

Țintă

Vomitoxin/Deoxynivalenol

Applications

Competitive ELISA

Assay type

Competitive (quantitative)

Sample type

Cereals, feed.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

Detection Limit is 10 ng/g.

Assay Time

<30 minutes

Recovery

75-105%

Principiul testului

Deoxynivalenol ELISA Test Kit (A327262) employs the competitive enzyme immunoassay technique for the quantitative measurement of Vomitoxin/Deoxynivalenol in cereals, feed. The 96-well microtiter plate has been pre-coated with Vomitoxin/Deoxynivalenol. During the incubation, Vomitoxin/Deoxynivalenol present in the samples or standards competes with the fixed amount of immobilized Vomitoxin/Deoxynivalenol for binding sites of the Anti-Vomitoxin/Deoxynivalenol Antibody, a HRP-Conjugated Secondary Antibody is added to the wells at the same time. The more Vomitoxin/Deoxynivalenol present in a sample or standard, the less Anti-Vomitoxin/Deoxynivalenol Antibody that binds to the plate. Following incubation, unbound HRP-Conjugated Secondary Antibody is removed by washing. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Vomitoxin/Deoxynivalenol present in each sample or standard. The concentration of Vomitoxin/Deoxynivalenol can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store at +4°C. Product is valid for 12 months. Do not use past expiration date.