Sulfadiazine ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Sulfadiazine

Applications

Competitive ELISA

Assay type

Competitive (quantitative)

Sensitivity

0.2 ppb

Sample type

Eggs, fish, shrimp, meat (beef, chicken and pork), honey, milk, milk powder.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Assay Time

<1 hour

Recovery

80-105%

Principiul testului

Sulfadiazine ELISA Test Kit (A327250) employs the competitive enzyme immunoassay technique for the quantitative measurement of Sulfadiazine in eggs, fish, shrimp, meat (beef, chicken and pork), honey, milk, milk powder. The 96-well microtiter plate has been pre-coated with Sulfadiazine. During the incubation, Sulfadiazine present in the samples or standards competes with the fixed amount of immobilized Sulfadiazine for binding sites of the Anti-Sulfadiazine Antibody, a HRP-Conjugated Secondary Antibody is added to the wells at the same time. The more Sulfadiazine present in a sample or standard, the less Anti-Sulfadiazine Antibody that binds to the plate. Following incubation, unbound HRP-Conjugated Secondary Antibody is removed by washing. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Sulfadiazine present in each sample or standard. The concentration of Sulfadiazine can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store at +4°C. Product is valid for 12 months. Do not use past expiration date.