Universal Ovalbumin Specific IgG ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Ovalbumin Specific IgG

Applications

Sandwich ELISA

Reactivity

Universal

Recommended dilutions

Serum and plasma samples may affect test results and should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Validated sample dilutions include Immunized mouse serum (1:1000-1:5000) and Healthy serum (Mouse) (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

0.938 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for Ovalbumin Specific IgG with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

1.563-100 ng/ml

Assay Time

4 hours

Principiul testului

Universal Ovalbumin Specific IgG ELISA Kit (A303886) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of universal Ovalbumin Specific IgG in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Ovalbumin Specific IgG binds to the immobilized antigen. Following incubation, unbound components are removed by washing and a biotinylated antigen detection reagent is added to the wells. This reagent binds the captured Ovalbumin Specific IgG to form an antigen-antibody-antigen sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Ovalbumin Specific IgG captured in each well. The concentration of Ovalbumin Specific IgG is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.