Rat Ovalbumin Specific IgG ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Ovalbumin Specific IgG

Applications

Indirect ELISA

Reactivity

Rat

Recommended dilutions

Serum and plasma samples may affect test results and should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Validated sample dilutions include Immunized rat serum (1:1000-1:5000) and Healthy serum (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

0.188 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for Ovalbumin Specific IgG with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.313-20 ng/ml

Assay Time

3 hours

Principiul testului

Rat Ovalbumin Specific IgG ELISA Kit (A303745) is a 3 hour indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of rat Ovalbumin Specific IgG in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Ovalbumin Specific IgG binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an enzyme-conjugated detection antibody is added to the wells. This detection antibody binds the captured Ovalbumin Specific IgG to form an indirect immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Ovalbumin Specific IgG present in the sample. The concentration of Ovalbumin Specific IgG is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.