Universal Heme ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Heme

Applications

Enzyme Catalysis Assay

Reactivity

Universal

Assay type

Enzyme catalysis (quantitative)

Sensitivity

5 pmol/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for Heme with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

15.6-1000 pmol/ml

Assay Time

30 Minutes.

Principiul testului

Universal Heme ELISA Kit (A303887) is a 2 hour enzyme catalysis assay designed for the in vitro quantitative determination of universal Heme in serum, plasma, tissue homogenates, and other biological fluids. This assay utilizes an enzyme catalysis detection method in which Heme present in the sample participates in a catalytic reaction to generate a coloured product. The intensity of the colour produced is directly proportional to the concentration of Heme in the sample. Absorbance is measured using a colorimetric microplate reader and the concentration of Heme is determined by comparison with a standard curve. This assay has a detection range of 15.6–1000 pmol/mL with a sensitivity of 5 pmol/mL.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.