Uncoated Rat IL1R1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

5 x 96T

Țintă

IL1R1

Applications

Sandwich ELISA

Reactivity

Rat

Assay type

Sandwich (quantitative)

Sample type

Serum and plasma

Cross reactivity

Specific for IL1R1 with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

78.13 - 5000 pg/ml

Assay Time

24 hours

Principiul testului

The Uncoated Rat IL1R1 ELISA Kit (A327170) is based on a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of IL1R1 in rat serum and plasma. In this format, a capture antibody specific to IL1R1 is first coated onto the wells of a 96-well microtiter plate by the user. After blocking nonspecific binding sites, standards or test samples are added, and IL1R1 present in the samples binds to the immobilized capture antibody. Following incubation, a biotinylated detection antibody specific for a different epitope of IL1R1 is introduced, forming a capture antibody–antigen–detection antibody complex. After washing to remove unbound detection antibody, HRP–streptavidin conjugate is added, binding to the biotinylated detection antibody. Unbound conjugate is removed by washing, and TMB substrate is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which changes to yellow after the addition of an acidic stop solution. The color intensity, measured at 450 nm, is directly proportional to the concentration of IL1R1 in the sample. Sample concentrations are determined by comparing their absorbance values to a standard curve generated from known IL1R1 standards.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.