Uncoated Human Hepatitis B Surface Antigen ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

5 x 96T

Țintă

Hepatitis B Surface Antigen

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sample type

Serum and plasma

Cross reactivity

Specific for Hepatitis B Surface Antigen with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.31-20 ng/ml

Assay Time

24 hours

Principiul testului

The Uncoated Human Hepatitis B Surface Antigen (HBsAg) ELISA Kit (A327165) is based on a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of HBsAg in human serum and plasma. In this format, a capture antibody specific to HBsAg is first coated onto the wells of a 96-well microtiter plate by the user. After blocking nonspecific binding sites, standards or test samples are added, and HBsAg present in the samples binds to the immobilized capture antibody. Following incubation, a biotinylated detection antibody specific for a different epitope of HBsAg is introduced, forming a capture antibody–antigen–detection antibody complex. After washing to remove unbound detection antibody, HRP–streptavidin conjugate is added, binding to the biotinylated detection antibody. Unbound conjugate is removed by washing, and TMB substrate is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which changes to yellow after the addition of an acidic stop solution. The color intensity, measured at 450 nm, is directly proportional to the concentration of HBsAg in the sample. Sample concentrations are determined by comparing their absorbance values to a standard curve generated from known HBsAg standards.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.