Acrylamide-ES ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Acrylamide-ES

Applications

Competitive ELISA

Assay type

Competitive (quantitative)

Sensitivity

2.5 ng/g or ppb

Sample type

Food samples such as potato/corn chips, french fries, cereals, and breads.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Assay Time

<1.5 hours

Recovery

75-95%

Principiul testului

Acrylamide-ES ELISA Test Kit (A327173) employs the competitive enzyme immunoassay technique for the quantitative measurement of Acrylamide-ES in food samples such as potato/corn chips, french fries, cereals, and breads. The 96-well microtiter plate has been pre-coated with Acrylamide-ES. During the incubation, Acrylamide-ES present in the samples or standards competes with the fixed amount of immobilized Acrylamide-ES for binding sites of the Anti-Acrylamide-ES Antibody, a HRP-Conjugated Secondary Antibody is added to the wells at the same time. The more Acrylamide-ES present in a sample or standard, the less Anti-Acrylamide-ES Antibody that binds to the plate. Following incubation, unbound HRP-Conjugated Secondary Antibody is removed by washing. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Acrylamide-ES present in each sample or standard. The concentration of Acrylamide-ES can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store at +4°C. Product is valid for 12 months. Do not use past expiration date.