Nitroimidazole ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Nitroimidazole

Applications

Competitive ELISA

Assay type

Competitive (quantitative)

Sample type

Tissue (chicken, duck, liver, fish, shrimp), egg, honey.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Assay Time

<5 hours

Recovery

75-115%

Principiul testului

Nitroimidazole ELISA Test Kit (A327234) employs the competitive enzyme immunoassay technique for the quantitative measurement of Nitroimidazole in tissue (chicken, duck, liver, fish, shrimp), egg, honey. The 96-well microtiter plate has been pre-coated with Nitroimidazole. During the incubation, Nitroimidazole present in the samples or standards competes with the fixed amount of immobilized Nitroimidazole for binding sites of the Anti-Nitroimidazole Antibody, a HRP-Conjugated Secondary Antibody is added to the wells at the same time. The more Nitroimidazole present in a sample or standard, the less Anti-Nitroimidazole Antibody that binds to the plate. Following incubation, unbound HRP-Conjugated Secondary Antibody is removed by washing. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Nitroimidazole present in each sample or standard. The concentration of Nitroimidazole can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store at +4°C. Product is valid for 12 months. Do not use past expiration date.