Melamine ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Melamine

Applications

Competitive ELISA

Assay type

Competitive (quantitative)

Sensitivity

1 ng/g or ppb

Sample type

Milk, yogurt, milk powder, egg, meat, fish, feed and other food samples.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Assay Time

<1 hour

Recovery

>80%

Principiul testului

Melamine ELISA Test Kit (A327226) employs the competitive enzyme immunoassay technique for the quantitative measurement of Melamine in milk, yogurt, milk powder, egg, meat, fish, feed and other food samples. The 96-well microtiter plate has been pre-coated with Melamine. During the incubation, Melamine present in the samples or standards competes with the fixed amount of immobilized Melamine for binding sites of the Anti-Melamine Antibody, a HRP-Conjugated Secondary Antibody is added to the wells at the same time. The more Melamine present in a sample or standard, the less Anti-Melamine Antibody that binds to the plate. Following incubation, unbound HRP-Conjugated Secondary Antibody is removed by washing. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Melamine present in each sample or standard. The concentration of Melamine can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store at +4°C. Product is valid for 12 months. Do not use past expiration date.