Mouse CCL20 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

CCL20

Applications

Sandwich ELISA

Reactivity

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

5.82 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

31.25 - 2000 pg/ml

Recovery

90% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant mouse CCL20.

Principiul testului

Mouse CCL20 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CCL20 in the samples. A monoclonal antibody specific for mouse CCL20 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CCL20, and CCL20 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL20 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!