Human CCL20 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

CCL20

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

8.55 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

15.63 - 1000 pg/ml

Recovery

101% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant human CCL20.

Principiul testului

Human CCL20 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CCL20 in the samples. A monoclonal antibody specific for human CCL20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and CCL20 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for CCL20 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of CCL20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage

Store at +4°C. Do not use past expiration date!