Mouse CCL2 ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

CCL2

Applications

Sandwich ELISA

Reactivity

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

20.54 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

62.50 - 4000 pg/ml

Recovery

100% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant mouse CCL2.

Principiul testului

Mouse CCL2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CCL2 in the samples. A monoclonal antibody specific for mouse CCL2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CCL2, and CCL2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!