Human SARS-CoV-2 (S) IgM ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

SARS-CoV-2 (S) IgM

Applications

Indirect ELISA

Reactivity

Human

Assay type

Indirect (qualitative)

Sensitivity

0.06 ng/ml

Sample type

Serum and plasma

Cross reactivity

Specific for SARS-CoV-2 (S) IgM with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Assay Time

2h 30m

Principiul testului

Human SARS-CoV-2 (S) IgM ELISA Kit (A327002) employs an indirect Enzyme-Linked Immunosorbent Assay (ELISA) technique for the qualitative detection of human SARS-CoV-2 (S) IgM in serum and plasma. The microtiter plate provided in this kit has been pre-coated with purified SARS-CoV-2 (S) IgM antigen. Following addition of standards and test samples to the wells, SARS-CoV-2 (S) IgM antibodies present in the sample bind to the immobilized antigen. Following incubation and washing, HRP-conjugated secondary antibody working solution is added to develop the antigen-antibody-secondary antibody immune complex. Following a further washing step to remove unbound components, TMB substrate solution is added to each well to visualize the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow following addition of acidic stop solution. The optical density (O.D.) is measured spectrophotometrically at 450 nm. Sample results are interpreted by comparison with the cut-off value to determine the presence or absence of SARS-CoV-2 (S) IgM antibodies in the tested samples.

Storage

Shipped at +4°C. Store kit components at +4°C as instructed in the Protocol Manual. Do not use past expiration date!