Imagine indisponibilăFree Testosterone ELISA Kit
Pe scurt
Free Testosterone ELISA Kit este un kit ELISA de tip Competitive (quantitative) pentru măsurarea Free Testosterone, cu reactivitate declarată pentru Universal. Măsoară în domeniul 31.25 - 2000 pg/ml, are o sensibilitate de 18.75 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma and other biological fluids. Se livrează în mărimea 96T, la prețul de 3.227,68 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Free Testosterone ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of Free Testosterone in serum, plasma, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Free Testosterone
- Applications
- Competitive ELISA
- Reactivity
- Universal
- Assay type
- Competitive (quantitative)
- Sensitivity
- 18.75 pg/ml
- Sample type
- Serum, plasma and other biological fluids.
- Cross reactivity
- Specific for Free Testosterone with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 31.25 - 2000 pg/ml
- Assay Time
- 2 hours
- Principiul testului
- Free Testosterone ELISA Kit (A326996) employs the competitive enzyme immunoassay technique for the quantitative measurement of Free Testosterone in serum, plasma, and other biological fluids. The 96-well microtiter plate has been pre-coated with Free Testosterone antigen. During the incubation, Free Testosterone present in the samples or standards competes with the fixed amount of immobilized Free Testosterone for binding sites on the Biotinylated Anti-Free Testosterone Antibody. The more Free Testosterone present in a sample or standard, the less Biotinylated Anti-Free Testosterone Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Free Testosterone Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Free Testosterone present in each sample or standard. The concentration of Free Testosterone can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
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3.227,68 lei2.667,50 leiTVA inclus (21%)fără TVA