Human CCL5 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

CCL5

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

0.53 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

15.63 - 1000 pg/ml

Recovery

97% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant human RANTES.

Principiul testului

Human CCL5 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human RANTES in the samples. A monoclonal antibody specific for human RANTES has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for RANTES, and RANTES present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of RANTES bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!