Human CTGF ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

CTGF

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

2.31 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

62.50 - 4000 pg/ml

Recovery

103% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant human CTGF.

Principiul testului

Human CTGF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CTGF in the samples. A monoclonal antibody specific for human CTGF has been immobilized onto microwells. Standard or samples are pipetted into the wells, and CTGF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for CTGF is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CTGF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!