4-Hydroxynonenal ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

4-Hydroxynonenal

Applications

Competitive ELISA

Reactivity

Universal

Assay type

Competitive (quantitative)

Sensitivity

0.38 ng/ml

Sample type

Serum, plasma and other biological fluids.

Cross reactivity

Specific for 4-Hydroxynonenal with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.63 - 40 ng/ml

Assay Time

2 hours

Principiul testului

4-Hydroxynonenal ELISA Kit (A326989) employs the competitive enzyme immunoassay technique for the quantitative measurement of 4-Hydroxynonenal in serum, plasma, and other biological fluids. The 96-well microtiter plate has been pre-coated with 4-Hydroxynonenal antigen. During the incubation, 4-Hydroxynonenal present in the samples or standards competes with the fixed amount of immobilized 4-Hydroxynonenal for binding sites on the Biotinylated Anti-4-Hydroxynonenal Antibody. The more 4-Hydroxynonenal present in a sample or standard, the less Biotinylated Anti-4-Hydroxynonenal Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-4-Hydroxynonenal Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of 4-Hydroxynonenal present in each sample or standard. The concentration of 4-Hydroxynonenal can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.