Anti-Macrophages Antibody [BA4D5]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Macrophages

Applications

WB, IP, Flow Cytometry, IHC-Fr, IF

Reactivity

Porcine

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

FC 1: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Clonă

BA4D5

Imunogen

Porcine alveolar macrophages.

Specificitate

This antibody recognises porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.Some expression has also been observed on peripheral blood lymphocytes.The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.It is believed that clone BA4D5 may be specific for porcine CD68 although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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