Anti-Histone H3 (tri methyl Lys36) Antibody

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Histone H3

Applications

ChIP, WB

Reactivity

Human, Mouse, Broad

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

ChIP: 10 µg per ChIP, WB: 0.5 µg/ml - 2 µg/ml

Imunogen

A peptide containing the tri-methylated lysine 36 of human histone H3.

Specificitate

This antibody recognises Histone H3 when tri-methylated at lysine 36.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones can be mono-, di- or tri-methylated by histone methyltransferases. Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Methylation events that weaken the binding between histone tails and DNA lead to increased transcription because they make the DNA more accessible to transcription factor proteins and RNA polymerase. Methylation of histones is therefore crucial for the regulation of gene expression. Tri-methylation of histone H3 at lysine 36 has been shown to lead to transcriptional activation. Wide species cross-reactivity is expected from Rabbit anti Human tri-methyl-histone H3 (Lys36) antibody based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului