Anti-Histone H3 (tri methyl Lys27) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Histone H3

Applications

ChIP, WB, IF, IHC-P

Reactivity

Human, Broad

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

ChIP: 5 µl per ChIP - 10 µl per ChIP, IF: 2 µg/ml, IHC-P: 2 µg/ml, WB: 0.5 µg/ml - 2 µg/ml

Imunogen

A peptide including tri-methyl lysine 27 of histone H3.

Specificitate

This antibody recognises Histone H3 when tri-methylated at lysine 27.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones can be mono-, di- or tri-methylated by histone methyltransferases. Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Tri-methylation of lysine 27 of histone H3 usually results in transcriptional repression.Wide species cross-reactivity is expected from Rabbit anti Human tri-methyl-histone H3 (Lys27) antibody based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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