Anti-Histone H3 (acetyl Lys 27) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Histone H3

Applications

ChIP, WB, IF

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

ChIP: 10 µg per ChIP, IF: 1 µg/ml - 5 µg/ml, WB: 0.1 µg/ml - 1 µg/ml

Imunogen

A peptide including acetyled lysine 27 of histone H3.

Specificitate

This antibody recognises Histone H3 when acetylated at lysine 27.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions. Wide species cross-reactivity is expected from Rabbit anti Human histone H3 (Ac27) antibody based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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