Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

50µl

Țintă

ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187)

Applications

WB

Reactivity

Human, Mouse, Rat

Formulation

Supplied in Phosphate Buffered Saline with 0.05% ProClin 300 and 50% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

WB: 1:500-1:2,000

Imunogen

Phospho-specific peptide corresponding to residues surrounding Thr202/Tyr204 of human ERK1 and residues surrounding Thr185/Tyr187 of human ERK2.

Specificitate

This antibody recognises ERK1 when phosphorylated at threonine 202/tyrosine 204 and ERK2 when phosphorylated at threonine 185/tyrosine 187. ERK1 and ERK2 are members of the Mitogen Activated Protein Kinase (MAPK) family. MAPKs constitute a diverse set of signaling pathways. In the case of ERK a cascade is initiated by binding activation of a membrane bound receptor which causes activation of a small GTPase, RAS, which in turn activates the protein kinase activity of the RAF protein. RAF in turn phosphorylates and activates MEK. MEK induces phosphorylation of ERK1 at residues threonine 202/tyrosine 204 and ERK 2 at residues threonine 185/tyrosine 187. Phosphorylation of the residues corresponds to activation of ERK1/2 and mediation of its diverse biological functions including cell growth, differentiation, motility, and adhesion via its effects on transcription, translation, cytoskeletal rearrangement and cross talk with other signaling pathways.

Storage

Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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