Anti-CD32-Like Antibody [VPM63]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD32-Like

Applications

Flow Cytometry, IHC-Fr

Reactivity

Sheep

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Clonă

VPM63

Imunogen

Sheep macrophages.

Specificitate

This antibody recognises a cell surface antigen of 40-42 kDa which is similar to the human cell surface antigen designated as CD32. It has therefore been hypothesized that clone VPM63 may recognise a minor isoform of the ovine homologue of human CD32 (Hopkins et. al. 1996).CD32 is one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. CD32, also known as the Fc gamma RII, acts as a low affinity receptor for IgG. The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. Mouse anti Sheep CD32-Like, clone VPM63 has been shown to recognize an antigen expressed by macrophages and monocytes but not by lymphocytes and shows strong staining of alveolar macrophages in flow cytometry.In addition to clone VPM63, clone CCG36, an antibody directed against bovine CD32 but cross reacting with ovine CD32 is also available. Clone CCG36 is available in both unconjugated and conjugated formats.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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