Anti-CD16-Like Antibody [VPM64]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD16-Like

Applications

IP, Flow Cytometry, IHC-Fr, ELISA

Reactivity

Sheep

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

ELISA: 1:500, Flow Cytometry: 1:25 - 1:200, IHC-Fr: Neat, IP: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Clonă

VPM64

Imunogen

Sheep macrophages.

Specificitate

This antibody recognises a cell surface antigen of 65-80kDa which is similar to the human cell surface antigen designated as CD16. It has therefore been hypothesized that clone VPM64 may recognize the ovine homologue of human CD16 (Hopkins, J. et. al. 1996). Clone VPM64 recognises an antigen that is strongly expressed by ovine alveolar macrophages and peripheral blood granulocytes, and more weakly by monocytes and other tissue macrophages.In addition to clone VPM64, clone KD1, an antibody directed against human CD16 and cross reacting with ovine and bovine CD16 is also available from Bio-Rad. Clone KD1 is available purified and conjugated to FITC.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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