Anti-EpCAM Antibody [VU-1D9] - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

500µg

Țintă

EPCAM

Applications

WB, IHC-P, IHC-Fr, Flow Cytometry, IP

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:50 - 1:100, IHC-P: 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Molecular weight

Approximately 38 kDa in HT-29 cell lysates

Clonă

VU-1D9

Imunogen

HG9 cell line (small cell lung carcinoma).

Specificitate

This antibody recognises epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium

Storage

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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