Anti-CD20 Antibody [IGEL/6850R]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD20

Applications

WB, IHC-P

Reactivity

Human, Canine

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A chromatography.

Isotype

IgG

Recommended dilutions

WB: 2-4 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

~ 33, 37 kDa

Cross reactivity

This antibody is predicted to cross react with Monkey, Baboon, Gorilla, and Chimpanzee.

Clonă

IGEL/6850R

Imunogen

Synthetic peptide corresponding to the C terminus (cytoplasmic domain), around amino acids 266-297, of human CD20 protein. The exact sequence is proprietary.

Specificitate

This antibody recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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