Anti-CD20 Antibody [SPM494]

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

100µl · 500µl

Țintă

CD20

Applications

Flow Cytometry, IF, IHC-P

Reactivity

Human, Baboon, Monkey

Formulation

Supplied as an aliquot of tissue culture supernatant with 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: 2-5µg/million cells, IF: 1:100-1:200, IHC-P: 2-4 µg/ml

Molecular weight

~ 33, 37 kDa

Cross reactivity

This antibody does not cross react with Bovine, Canine, Porcine, or Rat.

Purity

Tissue culture supernatant.

Light chain

kappa

Clonă

SPM494

Imunogen

Human tonsil B cells.

Specificitate

This antibody recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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