Anti-CD11b Antibody [M1/70.15] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD11b

Applications

Flow Cytometry

Reactivity

Mouse, Human, Rabbit

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Rat

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent.

Clonă

M1/70.15

Imunogen

T cell enriched splenocytes from B10 mice.

Specificitate

This antibody recognises the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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